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. 2019 Nov 29;9:17888. doi: 10.1038/s41598-019-54465-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of a single target in a MCDA reaction. Two sets of MCDA primers targeting the pgaD (a1,b1,c1,d1) and bla_OXA-23-like (a2,b2,c2,d2) genes were used in different reactions and the serial dilutions (10 ng, 10 pg, 1 pg, 100 fg, 10 fg and 1 fg) of target templates were subjected to MCDA reactions. (a1) and (a2), real-time turbidity applied for analysis of pgaD- and bla_OXA-23-like-MCDA products. (b1) and (b2), MG applied for analysis of pgaD- and bla_OXA-23-like-MCDA products. (c1) and (c2), LFB applied for analysis of pgaD- and bla_OXA-23-like-MCDA products. (d1) and (d2), gel electrophoresis applied for analysis of pgaD- and bla_OXA-23-like-MCDA products. Signals/Tubes/Biosensors/Lanes 1–6: A. baumannii (SG-AB001) genomic templates (10 ng-1fg); Signal/Tube/Biosensor/Lane 7: negative control (K. pneumoniae); Signal/Tube/Biosensor/Lane 8: blank control (DW). The d1 and d2 were cropped from different gels, the full-length gels can be found as Supplementary Fig. S1.