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. 2019 Nov 29;9:17888. doi: 10.1038/s41598-019-54465-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Specificity of duplex MCDA-LFB assay using different bacterial strains. The m-MCDA amplifications were carried out using different genomic DNA templates and the results were indicated using LFB. Biosensors 1–9, A. baumannii strains with bla_OXA-23-like gene; Biosensors 10–12, A. baumannii strains without bla_OXA-23-like gene; biosensors 13–27, Listeria monocytogenes, Bacillus cereus, Citrobacter braakii, Citrobacter freundii, Corynebacterium ammoniagenes, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Providencia rettgeri, Pseudomonas aeruginosa, Serratia marcescens, Serratia marcescens, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus salivarius.