. 2019 Nov 29;9:17888. doi: 10.1038/s41598-019-54465-8

Table 1.

Bacterial strains used in the study.

Strain ID	Strains	Sources of strains (No. of strains)^a	Duplex MCDA-LFB ^b
Strain ID	Strains	Sources of strains (No. of strains)^a	pgaD	bla_OXA-23-like
1	Acinetobacter baumannii	SG-AB001	P	P
2–50	Acinetobacter baumannii	Isolated strain(49)	P	P
51–53	Acinetobacter baumannii	Isolated strains (3)	P	N
54	Listeria monocytogenes	Isolated strains (1)	N	N
55	Bacillus cereus	Isolated strain (1)	N	N
56	Citrobacter braakii	Isolated strain (1)	N	N
57	Citrobacter freundii	Isolated strain (1)	N	N
58	Corynebacterium ammoniagenes	Isolated strain (1)	N	N
59	Escherichia coli	ATCC35218	N	N
60	Klebsiella pneumoniae	ATCC2146	N	N
61	Proteus mirabilis	Isolated strain (1)	N	N
62	Providencia rettgeri	Isolated strain (1)	N	N
63	Pseudomonas aeruginosa	ATCC27853	N	N
64	Serratia marcescens	Isolated strain (1)	N	N
65	Serratia marcescens	Isolated strain (1)	N	N
66	Staphylococcus aureus	ATCC29213	N	N
67	Staphylococcus epidermidis	Isolated strain (1)	N	N
68	Streptococcus salivarius	Isolated strain (1)	N	N

^aATCC, American Type Culture Collection; SG, Shougang Hospital; AB, Acinetobacter baumannii; ^bP, positive; N, negative. Only these strains belonging to the A. baumannii could be detected by the MCDA-LFB technique, indicating the extremely high selectivity of the method. Particularly, pgaD and bla_OXA-23-like genes can be simultaneously detected and correctly identified in a reaction vessel.