Table 3.
Primers used in the report.
| Gene | Primersa | Sequences and modifications (5′-3′)b | Lengthc |
|---|---|---|---|
| pgaD | p-F1 | GCTGCTTTTTCCACTCGTT | 19 nt |
| p-CP1 | CACTTCTGAAACATGATCGACCCTTATGGATTTTTGAAGGGCAC | 44 mer | |
| p-C1 | CACTTCTGAAACATGATCGACCC | 23 nt | |
| p-C1* | FITC-CACTTCTGAAACATGATCGACCC | 28 nt | |
| p-D1 | ACACATAGTCATAAATGAG | 19 nt | |
| p-R1 | CCTATTAACAGAAGTAAATGCA | 22 nt | |
| p-R2 | TCTGAGCGCGCTCATTT | 17 nt | |
| p-D2 | GATGATCGTCGCAGCAA | 17 nt | |
| p-C2 | ATGGGCAAGTTATAACTGGCTTAGA | 25 nt | |
| p-CP2 | ATGGGCAAGTTATAACTGGCTTAGAAACAGAGCTATTCGGGGC | 43 mer | |
| p-F2 | ATTGTGAGGCCAGCAACT | 18 nt | |
| blaOXA-23-like | b-F1 | TTCTGACCGCATTTCCAT | 18 nt |
| b-CP1 | CACAAGTGGGCTGGTTGACCCAAAAGCGACAATTTTTCCATC | 42 mer | |
| b-C1 | CACAAGTGGGCTGGTTGACC | 20 nt | |
| b-C1* | Dig-CACAAGTGGGCTGGTTGACC | 24 nt | |
| b-D1 | CTGGGTTGAGCAGCCA | 16 nt | |
| b-R1 | ACTGGTTGGGCAATGGA | 17 nt | |
| b-R2 | TAGCCATTACTCTCTTCT | 18 nt | |
| b-D2 | AAATGGAAGCTGTGTATGT | 19 nt | |
| b-C2 | TTACATTAGCCTGCACTTTTTCACT | 25 nt | |
| b-CP2 | TTACATTAGCCTGCACTTTTTCACTAGAGTTTGTTTCCCAATTAGC | 46 mer | |
| b-F2 | GTTACGCCTATTCAAGAGGT | 20 nt |
ap, pgaD gene; b, blaoxA-23-like gene; p-C1*, 5′-labeled with FITC when used in MCDA-LFB assay; b-C1*, 5′-labeled with Dig when used in MCDA-LFB assay;
bFITC, fluorescein isothiocyanate; Dig, digoxigenin;
cmer, monomeric unit; nt, nucleotide.