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. 2019 Nov 29;10:5462. doi: 10.1038/s41467-019-13382-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — IF validation of markers derived from the single-cell data in mature kidney organoids. a IF staining of an entire kidney organoid with segment specific markers as shown. b Schematic of kidney nephron with major cell types and canonical markers annotated. c Immunofluorescence staining of D29 kidney organoids for podocyte (WT1), proximal tubule (LTL), and distal tubule (CDH1 and GATA3) across two protocols (JB, ML) and four cell lines (AS, N1, N2, ThF). IF staining for validation of markers identified in the single-cell data: d NPHS1 colocalized with the podocyte-specific marker SYNPO and e LRP2 colocalized with the proximal tubular marker LTL (bottom). f IF staining validation for MEIS1-positive mesenchymal cells in D29 organoids. LAMA1 indicates basement membranes. MEIS1 staining of mesenchymal cells appropriately surrounds LAMA1-defined tubular nephron structures.