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. 2019 Nov 29;10:5462. doi: 10.1038/s41467-019-13382-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Transplantation of human organoids into mouse diminishes off-target cells. t-SNE plots of organoids in prolonged in vitro culture reveal cell clusters in a D32 and b D51 organoids similar to clusters from D29 organoids. c Transplanted and vascularized organoid at D51. IF staining shows human podocytes (anti-human NPHS1 antibody) at the outer perimeter and mouse endothelial cells (anti-mouse Plvap antibody) lining the internal perimeter of a glomerular structure. Gray, human and mouse nuclei (DAPI). d Dotplots indicating ligand and receptor pairs involved in putative cross-talk between human podocytes and mouse endothelial cells of transplanted organoids. e Violin plots of expression levels for KLF6 and HES1 in the distal MAL+ cluster from D29, D32 and D51 organoids grown in vitro, compared to D32 transplanted organoids. f Violin plots demonstrate that PMEL+ melanoma cells and SOX2+ neuronal cells were diminished after transplantation. MYOG+ muscle cells and STMN2+ neuron-like cells persisted. g Random Forest Classifier shows the relation of cell clusters in transplant organoids compared to organoids grown in vitro. Melanoma and SOX2+ neuronal precursor cells are not detected in D32 transplant organoids. h IF staining validation for SOX2 (green) in D32 control organoids compared to diminished abundance in D32 transplanted organoids. Human and mouse nuclei (DAPI, gray).