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. 2019 Nov 29;9:17992. doi: 10.1038/s41598-019-54312-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HDAC4 and HDAC5 deacetylate TBX5 and promote its nuclear export. (A,B) Pull-down and Western blot analysis showing that both HDAC4 and HDAC5 strongly deacetylate TBX5. KAT2B was used to promote TBX5 acetylation. Full-length blots are shown in Supplementary Fig. S2 (A–D). (C) Representative images of the cellular distribution of TBX5 following HDAC4/HDAC5 overexpression, showing the partial re-localization of TBX5 into the cytoplasm. (D) Cell count showing the cellular distribution of TBX5 in cells transfected with TBX5 alone or TBX5 and HDAC4/5 (TBX5- total counts 300; 292 (N) and 8 (N + C), TBX5 + HDAC4- total cell counts 257; 198(N) and 59 (N + C) and TBX5 + HDAC5− total counts 274; 195(N) and 79(N + C). Results are from three individual experiments (N = nuclear, C = cytoplasmic).