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. 2019 Nov 29;10:5463. doi: 10.1038/s41467-019-13237-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — IRP1 localizes to nuclei. a Protoporphyrin accumulation/autofluorescence in prothoracic glands (PG) from IRP1A-RNAi (IRP1A^IR) flies reared on iron-depleted (=BPS) media for three generations. Adult survival relative to last larval stage (surviving third instar larvae = L3 = 100%). Scale bar = 250 μm. b Protoporphyrin accumulation/autofluorescence in PGs from IRP1A^FCF animals (tissue-specific excision of IRP1A, Supplementary Fig. 4) reared on iron-rich medium until L2, after which larvae were switched to BPS-supplemented food. Scale bar = 250 μm. c Survival of IRP1A and IRP1B null mutants (KO = knockout, Supplementary Fig. 4). Error bars represent standard deviation from three biological replicates (each sample contained 50 individuals). d Subcellular localization of PG-specific, Flag-tagged IRP1A and IRP1B (PG > IRP1A/PG > IRP1B transgenic lines, Supplementary Table 1). DAPI was used to stain DNA/nuclei. Scale bar = 250 μm. e Subcellular localization of Flag-tagged transgenic human IRP1 and IRP2 (PG > hIRP1 and PG > hIRP2, Supplementary Table 1) expressed specifically in the PG. Scale bar = 250 μm. f Subcellular localization of Flag-tagged proteins encoded by IRP1A^3F and IRP1B^3F knock-in alleles (Supplementary Fig. 4) in control or AGBE mutant backgrounds (AGBE^+/+ = PG > FLP. AGBE^−/− = PG > FLP; AGBE^FCM, Supplementary Fig. 4). Scale bar = 500 μm. g Subcellular localization of Flag-tagged proteins encoded by IRP1A^3F and IRP1B^3F knock-in alleles (Supplementary Fig. 4) in the fat body (FB) and salivary gland (SG). Scale bar = 500 μm. Source data are provided as a Source Data file.