Table 1.

Transcriptional responses of expressing IRP1A alleles in the prothoracic gland.

Rank (n/234)	Symbol	Description	FC 3R3Q:C	P	FC IRP1A:C	P	FC C450S:C	P
6	dib	Ecdysone biosynthesis/heme binding/P450	−13.7	4.0E-03	−2.4	>0.05	−1.7	>0.05
7	phm	Ecdysone biosynthesis/heme binding/P450	−11.7	1.7E-02	−2.2	>0.05	1.1	>0.05
12	sad	Ecdysone biosynthesis/heme binding/P450	−9.7	3.2E-02	−1.7	1.7E-02	1.2	>0.05
13	Start1	Sterol transport	−9.6	4.7E-03	−3.1	3.3E-02	−2.3	>0.05
15	CG7322	Short-chain dehydrogenase	−9.0	1.4E-02	−1.8	>0.05	−1.1	>0.05
23	Cyp6g2	Heme binding/P450	−7.2	1.7E-02	−1.1	>0.05	−1.2	>0.05
26	spidey	Short-chain dehydrogenase	−7.0	1.8E-02	−1.6	2.9E-02	−1.6	1.6E-02
34	nvd	Ecdysone biosynthesis/iron sulfur cluster	−6.1	1.7E-02	−2.1	8.9E-03	−1.6	>0.05
35	sro	Ecdysone biosynthesis/short-chain dehydrogenase	−6.0	2.1E-03	−4.2	1.3E-02	−1.2	>0.05
41	GstE14	Ecdysone biosynthesis/glutathione S transferase	−5.6	2.8E-02	−2.6	>0.05	−1.4	>0.05
44	ND-15	NADH:ubiquinone oxidoreductase, iron-sulfur subunit 5	−5.2	2.8E-02	−3.3	>0.05	−2.7	8.9E-03
47	ouib	Ecdysone biosynthesis/zinc finger	−4.9	2.4E-02	−2.1	>0.05	−1.8	>0.05
51	spok	Ecdysone biosynthesis/heme binding/P450	−4.8	3.6E-03	−2.4	2.9E-03	−1.2	>0.05
52	scu	Short-chain dehydrogenase/reductase	−4.7	1.1E-02	−1.3	>0.05	1.2	>0.05
55	Cyt-b5	Cytochrome b5-like heme/steroid binding domain	−4.5	4.4E-02	−1.8	>0.05	−1.4	1.0E-02
56	CG17928	Cytochrome b5-like heme/steroid binding domain	−4.5	1.4E-02	−1.8	>0.05	−2.3	3.0E-03
64	Tig	Heme oxygenase-like	−4.2	6.0E-03	−1.7	>0.05	−1.2	>0.05
74	ND-19	NADH:ubiquinone oxidoreductase	−3.9	4.0E-02	−1.5	>0.05	−1.2	>0.05
98	Fdx2	Ferredoxin/iron sulfur cluster assembly	−3.4	1.3E-03	−1.6	>0.05	1.3	>0.05
107	Npc2a	Sterol transport	−3.3	8.4E-03	−1.9	>0.05	−1.6	>0.05
116	Pbgs	Heme biosynthesis	−3.2	6.4E-03	−1.5	>0.05	−1.2	>0.05
125	Npc1a	Sterol transport	−3.1	3.2E-04	−2.3	2.4E-02	−1.2	>0.05
146	Drat	Response to hypoxia	−2.9	1.6E-02	−1.4	>0.05	−1.6	1.1E-02
170	CG31548	Short-chain dehydrogenase/reductase	−2.7	3.1E-02	−1.8	>0.05	−1.4	>0.05
173	CG32857	Nfu1 homolog/iron sulfur cluster assembly	−2.7	3.7E-02	−1.2	>0.05	−1.2	>0.05
184	ance	Ecdysone biosynthesis/zinc finger	−2.7	3.7E-02	−2.0	>0.05	−1.2	>0.05
202	Alas	Heme biosynthesis	−2.6	1.5E-02	−1.0	>0.05	12.9	2.6E-04
212	CG12056	Cytochrome b5-like heme/steroid binding domain	−2.6	1.7E-02	−1.5	>0.05	1.1	>0.05
218	CG2254	Short-chain dehydrogenase/reductase	−2.6	6.7E-03	1.2	5.1E-05	−1.1	>0.05
221	Vhl	Response to hypoxia	−2.6	2.0E-02	−1.6	>0.05	1.1	>0.05

RNA-Seq analysis of prothoracic gland (PG) samples with PG-specific expression of IRP1A^3R3Q (non-RNA-binding and nuclear), wild type IRP1A and IRP1A^C450S (constitutively RNA-binding and largely cytoplasmic) transgenes (Supplementary Table 1). A total of 234 genes were significantly downregulated by IRP1A^3R3Q expression, using a cutoff of −2.5-fold and P < 0.05. The fold changes (relative to control = C) and P-values (t-test) are shown for all three conditions and sorted by relative fold changes of IRP1A^3R3Q vs. controls. For all 234 genes, see Supplementary Data 3, for term enrichment in this set, see Table 2 and Supplementary Data 4