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. 2019 Nov 29;9:17897. doi: 10.1038/s41598-019-52872-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ischemic stroke alters kidney function. (A) TTC staining images of sham and ischemic stroke-induced (MCAO) rat brain. (B) Estimation of albumin and creatinine levels in MCAO rats. Error bars indicate mean ± SE; n = 6. ****p < 0.0001. (C) Urine samples from sham (S) and stroke-induced rats (M) were subjected to SDS-PAGE and urinary proteins were visualized by silver staining, Mr, molecular weight marker (#1610374; Bio-Rad); BSA, Bovine serum albumin. HIF1α expression in the infarcted region of the brain (D) and glomerular lysates (E) from sham and MCAO rats. Densitometric analysis of HIF1α band is depicted after normalized for respective β-actin expression (F). Error bars indicate mean ± SE; n = 5–6. ****p < 0.0001. (G) Steady-state mRNA levels of HIF1α from the brain and glomerular lysates were measured by qRT-PCR. Error bars indicate mean ± SE; n = 6. ****p < 0.0001.