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. 2019 Nov 29;9:17897. doi: 10.1038/s41598-019-52872-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Essential role of ZEB2 in regulating TRPC6 expression: (A) Immunoblotting analysis of ZEB2, TRPC6, and pFAK expression in podocytes expressing siZEB2 and treated with or without FG-4592. (B) Quantification of band intensities of ZEB2, TRPC6, pFAK was performed with ImageJ software. Error bars indicate mean ± SE; n = 3, *p < 0.01, **p < 0.008, and ***p < 0.0002. (C) Immunoblotting analysis of TRPC6 and pFAK expression in podocytes in which TRPC6 expression was knocked-down and treated with or without FG-4592. (D) Quantification of band intensities of western blots was performed with ImageJ. Error bars indicate mean ± SE; n = 3, **p < 0.008 and ****p < 0.0001. (E) Immunoblotting analysis of TRPC6, pFAK, and RhoA expression in podocytes exposed to FG-4592 and treated with or without 2APB and FAKI14. (F) Quantification of band intensities of western blots was performed with ImageJ. Error bars indicate mean ± SE; n = 3, *p < 0.01 and ****p < 0.0001.