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. Author manuscript; available in PMC: 2020 Dec 15.
Published in final edited form as: Dev Biol. 2019 Aug 20;456(2):154–163. doi: 10.1016/j.ydbio.2019.08.012

Figure 3. PRMT5 is required for the survival of early patella progenitors.

Figure 3.

A-B. Early committed patella cells were visualized by SOX9 (red), SCX-GFP (green) expression at E13.5 in the knee joint of 4 control and 4 PrxCre;Prmt5c/c embryos. C-D. TUNEL staining (white) of apoptosing cells in E13.5 control and mutant knees. E-F. Visualization of differentiating patella progenitor cells in the E14.5 knee joint of 3 control and 3 PrxCre;Prmt5c/c embryos. G-H. TUNEL staining (white) of apoptosing cells in E14.5 knees. Nuclei stained with DAPI (blue). I. Percentage of TUNEL-positive cells in E13.5/E14.5 was quantified across 4 control and 4 mutant knees at E13.5 and across 3 control and 3 mutant knees at E14.5. J. Gene expression of Prmt5 and Bmp4 were measured through quantitative-RT-PCR in 3 control and 3 mutant knee joints at E14.5. *p<0.05; **p<0.01; ***p<0.001 using an unpaired Student’s t-test (Scale = 50μm).