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. Author manuscript; available in PMC: 2020 Dec 15.
Published in final edited form as: Dev Biol. 2019 Aug 20;456(2):154–163. doi: 10.1016/j.ydbio.2019.08.012

Figure 5. PRMT5 is required for later differentiation of cartilage cells.

Figure 5.

A,C. SOX9 protein expression (green) by immunofluorescence in the tibia of control and Col2Cre;Prmt5c/c mice at P10 (DAPI in blue). B,D. Visualization of RUNX2 expression (brown) by immunohistochemical staining in the tibia of control and Col2Cre;Prmt5c/c mice at P10. E,G. Visualization of Col2a1 expression (green) in the tibia of control and Col2Cre;Prmt5c/c mice at P10 by fluorescent in situ hybridization (DAPI in blue). F,H. Visualization of Mmp13 expression (green) in the tibia of control and Col2Cre;Prmt5c/c mice at P10 (DAPI in blue). Differentiation marker studies were performed on 3 control and 3 mutant knees at E18.5. GP-growth plate; PHC-pre-hypertrophic chondrocytes; HC-hypertrophic chondrocytes (Scale = 100μm).