Table 1:
List of antibody used.
| Antibody type | Target | Host Species | Concentration | Source |
|---|---|---|---|---|
| Primary antibodies | Bassoon | Rabbit | 1:1000 | Millipore ABN255 |
| Cannabinoid receptor 1 | Rabbit | 1:1000 | ImmunoGenes | |
| Tyrosine Hydroxylase | Mouse | 1:5000 | ImmunoStar 22941 | |
| Tyrosine Hydroxylase | Chicken | 1:1000 | Abcam ab76442 | |
| Vesicular Inhibitory Amino Acid Transporter (VIAAT) | Rabbit | 1:500 | Synaptic Systems 131 003 | |
| Vesicular Inhibitory Acid Transporter (VIAAT) | Guinea pig | 1:2000 | Synaptic Systems 131 004 | |
| Vesicular Glutamate Transporter 1 (vGluT1) | Guinea pig | 1:5000 | Synaptic Systems 135 304 | |
| Secondary antibodies | Alexa488-conjugated anti-mouse | Donkey | 1:400 | Jackson 715-545-150 |
| Alexa594-conjugated anti-rabbit | Donkey | 1:400 | Jackson 711-595-152 | |
| Alexa647-conjugated anti-rabbit | Donkey | 1:400 | Jackson 711-605-152 | |
| Alexa647-conjugated anti-guinea pig | Donkey | 1:400 | Jackson 706-605-148 | |
| CF568-conjugated anti-guinea pig | Donkey | 1:1000 | Biotium 20377 | |
| DyLight405-conjugated anti-mouse | Donkey | 1:400 | Jackson 715-475-151 |