Figure 8.

(a–f) Representative photomicrographs of microglia (primary antibody against OX‐42) in the paraventricular nucleus (PVN) of targeted Gαi₂ or control scrambled (SCR) intracerebroventricular oligodeoxynucleotide (ODN)‐infused [25 µg (5 µl)⁻¹ day⁻¹ for 7 days] and minocycline (MINO)–ODN co‐infused [ODN, 25 µg (5 µl)⁻¹ day⁻¹; MINO, 120 µg day⁻¹ for 7 days in HS only] male Sprague–Dawley rats on 7 days of normal‐salt (NS; 0.6% NaCl) or high‐salt (HS; 4% NaCl) diet. (a) SCR ODN‐infused rat on NS diet. (b) SCR ODN‐infused rat on HS diet. (c) SCR ODN‐infused rat on HS diet co‐infused with minocycline. (d) Gαi₂ ODN‐infused rat on NS diet. (e) Gαi₂ ODN‐infused rat on HS diet. (f) Gαi₂ ODN‐infused rat on HS diet co‐infused with MINO (scale bars: 200 µm, ×20 magnification). (g) Average number of PVN microglia in randomly selected 200 µm × 200 µm box in Sprague–Dawley rats during SCR or Gαi₂ ODN infusion maintained on NS or HS diet and minocycline–ODN co‐infusion on HS diet. Abbreviation: 3V, third ventricle. (n = 6 per group, ^* P < 0.05 versus NS in respective ODN treatment group; ^τ P < 0.05 versus HS in respective ODN treatment group, mean ± SD.)