Figure 9.

Representative photomicrographs of microglia in the paraventricular nucleus (PVN) of targeted Gαi₂ or control scrambled (SCR) intracerebroventricular oligodeoxynucleotide (ODN)‐infused [25 µg (5 µl)⁻¹ day⁻¹ for 7 days] and minocycline (MINO)–ODN co‐infused [ODN, 25 µg (5 µl)⁻¹ day⁻¹; MINO, 120 µg day⁻¹ for 7 days in HS only] male Sprague–Dawley rats on 7 days of normal‐salt (NS; 0.6% NaCl) or high‐salt (HS; 4% NaCl) diet. (a) SCR ODN‐infused rat on NS diet. (b) SCR ODN‐infused rat on HS diet. (c) SCR ODN‐infused rat on HS diet co‐infused with minocycline. (d) Gαi₂ ODN‐infused rat on NS diet. (e) Gαi₂ ODN‐infused rat on HS diet. (f) Gαi₂ ODN‐infused rat on HS diet co‐infused with minocycline. (g) Impact of minocycline co‐infusion (120 µg day⁻¹ for 7 days) on percentage activation of PVN microglia in Sprague–Dawley rats during SCR or Gαi₂ ODN infusion maintained on HS diet. (h) Impact of minocycline on number of PVN active microglia in Sprague–Dawley rats during SCR or Gαi₂ ODN infusion maintained on HS diet. (n = 6 per group, scale bars: 20 µm; green arrowheads indicate representative activated microglia; red arrowheads indicates representative inactivated microglia; ×40 magnification; ^* P < 0.05 versus NS in respective ODN treatment group; ^τ P < 0.05 versus HS in respective ODN treatment group, mean ± SD.)