Figure 12.

(a–f) Representative photomicrographs of microglia (primary antibody against OX‐42) in the subfornical organ (SFO) of targeted Gαi₂ or control scrambled (SCR) intracerebroventricular oligodeoxynucleotide (ODN)‐infused [25 µg (5 µl)⁻¹ day⁻¹ for 7 days] and minocycline (MINO)–ODN co‐infused [ODN, 25 µg (5 µl)⁻¹ day⁻¹; MINO, 120 µg day⁻¹ for 7 days in HS only] male Sprague–Dawley rats on 7 days of normal‐salt (NS; 0.6% NaCl) or high‐salt (HS; 4% NaCl) diet. (a) SCR ODN‐infused rat on NS diet. (b) SCR ODN‐infused rat on HS diet. (c) SCR ODN‐infused rat on HS diet co‐infused with minocycline. (d) Gαi₂ ODN‐infused rat on NS diet. (e) Gαi₂ ODN‐infused rat on HS diet. (f) Gαi₂ ODN‐infused rat on HS diet co‐infused with MINO. (g) Impact of minocycline co‐infusion (120 µg day⁻¹ for 7 days) on percentage activation of PVN microglia in Sprague–Dawley rats during SCR or Gαi₂ ODN infusion maintained on HS diet. (h) Impact of minocycline co‐infusion (120 µg day⁻¹ for 7 days) on number of active PVN microglia in Sprague–Dawley rats during SCR or Gαi₂ ODN infusion maintained on HS diet. (I) Impact of minocycline co‐infusion (120 µg day⁻¹ for 7 days) on total number of PVN microglia in Sprague–Dawley rats during SCR or Gαi₂ ODN infusion maintained on HS diet. (n = 6 per group, scale bar: 200 µm, ×20 magnification, mean ± SD.)