Table 3.
Comparison of T. cruzi DNA detection by means of T. cruzi satDNA/IRBP qPCR assay (index test) and standardized qPCR (comparator test)
| Panel of well-characterized samples | n | Index test | |
|---|---|---|---|
| Positive | Negative | ||
| Comparator qPCR test-positive | |||
| Canis familiaris | 2 | 2 | 0 |
| Felis catus | 5 | 5 | 0 |
| Ovis aries | 3 | 3 | 0 |
| Didelphis sp. | 6 | 6 | 0 |
| Total | 16 | 16 | 0 |
| Comparator qPCR test-negative | |||
| Canis familiaris | 9 | 0 | 9 |
| Felis catus | 1 | 0 | 1 |
| Ovis aries | 3 | 1 | 2 |
| Capra hircus | 9 | 0 | 9 |
| Promops nasutus | 6 | 0 | 6 |
| Conepatus chinga | 1 | 0 | 1 |
| Lagostomus maximus | 1 | 0 | 1 |
| Total | 30 | 1 | 29 |
Note: Comparator qPCR test was reported in Ramirez et al. [13]