Table 5.
Parasite loads in satDNA/IRBP qPCR-positive samples from mammalian reservoir hosts
| Sample ID | Mammalian species | Duplex SatDNA/IRBP qPCR | DTU | |||
|---|---|---|---|---|---|---|
| Mean Cq IRBP | Mean Cq cruzi | par.eq/ml | log par.eq/ml | |||
| 1 | Felis catus | 25.59 | 28.70 | 0.30 | − 0.522878745 | TcI |
| 2 | Felis catus | 27.13 | 28.52 | 0.36 | − 0.443697499 | TcI |
| 3 | Felis catus | 27.82 | 24.78 | 9.49 | 0.977266212 | TcI |
| 4 | Felis catus | 26.53 | 24.73 | 9.94 | 0.997386384 | TcI |
| 5 | Felis catus | 26.39 | 23.75 | 23.72 | 1.375114685 | TcI |
| 6 | Felis catus | 26.11 | 23.96 | 19.67 | 1.29380436 | TcI |
| 7 | Felis catus | 25.96 | 25.58 | 66.40 | 1.822168079 | TcI |
| 8 | Felis catus | 26.70 | 20.89 | 296.45 | 2.471951455 | TcI |
| 9 | Felis catus | 25.84 | 21.58 | 160.17 | 2.204581176 | TcI |
| 10 | Felis catus | 26.48 | 21.40 | 187.50 | 2.273001272 | TcI |
| 11 | Ovis ariesa | 31.61 | 29.45 | 0.14 | − 0.853871964 | TcI/TcVI |
| 12 | Ovis ariesa | 33.74 | 29.13 | 0.21 | − 0.677780705 | TcI/TcVI |
| 13 | Canis familiarisa | 29.60 | 27.47 | 0.84 | − 0.075720714 | TcVI |
| 14 | Canis familiarisa | 28.83 | 29.31 | 0.18 | − 0.744727495 | TcI/TcVI |
| 15 | Canis familiarisa | 24.69 | 29.27 | 0.19 | − 0.721246399 | TcI/TcVI |
| 16 | Canis familiaris | 35.92 | 18.23 | 402.73 | 2.60500859 | TcI |
| 17 | Didelphis marsupialis | 26.01 | 26.80 | 0.96 | − 0.017728767 | nd |
| 18 | Didelphis marsupialis | 26.54 | 21.29 | 46.41 | 1.666564777 | nd |
| 19 | Didelphis marsupialis | 26.30 | 22.04 | 27.39 | 1.437592032 | nd |
| 20 | Marmosa murina | 32.76 | 29.28 | 0.17 | − 0.782516056 | nd |
| 21 | Marmosa murina | 32.37 | 29.17 | 0.21 | − 0.688246139 | nd |
| 22 | Marmosa murina | 24.07 | 18.72 | 284.38 | 2.453891414 | nd |
a DNA extracted with phenol-chloroform
Abbreviation: nd, not determined