Fig. 3.

Evidence for functional activity of vasculature in bone implants. The spleen of NOD/SCID mice implanted with human bone was dissociated for the detection of human B cells disseminated (n = 5/group). Single-cell suspensions were labelled with antibodies specific for human IgG-PE and CD19-FITC and mouse CD45. FACS scatter plots were gated for all viable cells (R1), then mouse CD45-negative cells (R2). R5 from each spleen sample was plotted as a histogram for human CD19+ IgG+ cells for each time point. Data represents mean ± SEM, statistical significance determined by one-way ANOVA, *p < 0.01, **p < 0.001