Fig. 7.

IL-1B signalling drives breast cancer metastasis to bone. a Gene expression of IL-1B in human bone implants isolated from NOD SCID mice 4 weeks after intraductal injection of MDA-Td Tomato cells. Data shown are from human bone implants bones isolated from mice in which MDA-Td Tomato cells did not metastasise and from human bone implants with MDA-Td Tomato metastases compared with bone from the same patient isolated from a mouse not injected with breast cancer cells. Data shown are mean ± SEM for n = 5–10 mice per group. Statistical significance determined by one-way ANOVA, *p < 0.01, **p < 0.001, ***p < 0.0001. b Effects of inhibiting IL-1 signalling on metastasis to human bone implants. MDA Td Tomato cells were othrotopically injected into NOD SCID ϒ mice 4 weeks after implantation of human bone. Mice were randomised to receive 1 mg/kg/day anakinra or placebo. Percentage of animals with metastasis in human bone was quantified 8 weeks post tumour cell injection. Data shown are from 7 to 10 mice per group