Table 1.

Summary of all gene and genome editing reports in Y. lipolytica

Tool	Notes	Addgene	Refs
Cas9 indel and integration	Hybrid promoters combining strong RNA pol II promoters and tRNA lead to high efficiency indel	70007	[51]
Cas9 indels, multiplexed	Multiplexed indels by multiple sgRNAs processed with hammerhead ribozymes	73226	[52]
Cas9 indels	T7 polymerase expression of sgRNA; Addition of GGG improved sgRNA activity	N/A	[53]
Cas9 integration	Non-essential landing sites were established for markerless integration	84608-17	[27]
Cas9 deletion and integration	Two sgRNAs used to excise DNA regions. Simultaneous integration by HMEJ	N/A	[55]
Cas9 integration	EasyCloneYALI: integration of non-replicating constructs by HR at 11 landing sites in Ku70 knockout	100000 0140-141	[56]
TALENs indel and integration	TALEN induced DSB can create indels or mediate HR based integration	N/A	[57]
dCas9-MxiI	sgRNA targeted upstream of the TSS	91248	[58]
dCas9 dCpfI	Multiplexed targeting of both dCas9 and dCpfI a pathway	N/A	[59]
CRISPR-VPR	CRISPRa upregulated two genes for cellobiose metabolism	N/A	[60]
PiggyBac	TTAA specific transposon insertion; excision/integration mutant used for recycle selection markers	N/A	[66]
Hermes Tn	Sequence independent transposon insertion; useful for functional genomics and strain engineering	113332	[67]
Cas9 genome scale indel	Sixfold redundant sgRNA for entire genome; useful for functional genomics and strain engineering	N/A	[68]