Table 1.
Samples | Total no. of reads | No. of trimmed reads | Mapping with rainbow trout reference genome | Mapping with Atlantic salmon reference genome | Mapping with brown trout reference transcriptome | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. of mapped reads | % of mapped reads | No. of unmapped reads | % unmapped reads | No. of mapped reads | % of mapped reads | No. of unmapped reads | % unmapped reads | No. of mapped reads | % of mapped reads | No. of unmapped reads | % unmapped reads | |||
Control 1 | 37,453,251 | 37,432,308 | 28,587,181 | 76.37 | 8,845,127 | 23.63 | 31,555,799 | 84.3 | 5,876,509 | 15.7 | 33,957,176 | 90.72 | 3,475,132 | 9.28 |
Control 2 | 36,014,039 | 36,000,623 | 27,596,056 | 76.65 | 8,404,567 | 23.35 | 30,167,749 | 83.8 | 5,832,874 | 16.2 | 32,695,497 | 90.82 | 3,305,126 | 9.18 |
Control 3 | 33,756,491 | 33,742,632 | 25,599,517 | 75.87 | 8,143,115 | 24.13 | 28,588,179 | 84.72 | 5,154,453 | 15.28 | 30,631,472 | 90.78 | 3,111,160 | 9.22 |
Control 4 | 28,906,028 | 28,893,551 | 22,082,023 | 76.43 | 6,811,528 | 23.57 | 24,154,386 | 83.6 | 4,739,165 | 16.4 | 26,126,636 | 90.42 | 2,766,915 | 9.58 |
Control 5 | 35,936,239 | 35,917,599 | 27,065,039 | 75.35 | 8,852,560 | 24.65 | 30,203,792 | 84.09 | 5,713,807 | 15.91 | 32,698,686 | 91.04 | 3,218,913 | 8.96 |
Control 6 | 35,784,560 | 35,769,721 | 27,665,605 | 77.34 | 8,104,116 | 22.66 | 30,149,740 | 84.29 | 5,619,981 | 15.71 | 32,632,620 | 91.23 | 3,137,101 | 8.77 |
Exposed 1 | 36,037,693 | 36,021,748 | 26,712,983 | 74.16 | 9,308,765 | 25.84 | 30,604,349 | 84.96 | 5,417,399 | 15.04 | 32,234,190 | 89.49 | 3,787,558 | 10.51 |
Exposed 2 | 36,585,106 | 36,570,194 | 27,967,577 | 76.48 | 8,602,617 | 23.52 | 30,928,270 | 84.57 | 5,641,924 | 15.43 | 33,142,773 | 90.63 | 3,427,421 | 9.37 |
Exposed 3 | 34,790,815 | 34,777,577 | 26,339,581 | 75.74 | 8,437,996 | 24.26 | 29,540,271 | 84.94 | 5,237,306 | 15.06 | 31,484,762 | 90.53 | 3,292,815 | 9.47 |
Exposed 4 | 34,863,462 | 34,848,992 | 26,601,431 | 76.33 | 8,247,561 | 23.67 | 29,178,501 | 83.73 | 5,670,491 | 16.27 | 31,894,763 | 91.52 | 2,954,229 | 8.48 |
Exposed 5 | 35,989,820 | 35,973,511 | 27,781,132 | 77.23 | 8,192,379 | 22.77 | 30,067,422 | 83.58 | 5,906,089 | 16.42 | 32,541,899 | 90.46 | 3,431,612 | 9.54 |
Exposed 6 | 35,705,332 | 35,687,549 | 27,935,659 | 78.28 | 7,751,890 | 21.72 | 29,067,347 | 81.45 | 6,620,202 | 18.55 | 32,373,706 | 90.71 | 3,313,843 | 9.29 |
Notes: Filtered clean reads were mapped against the available genomes of rainbow trout and Atlantic salmon, and brown trout reference transcriptome