Fig. 4.

Epitope mapping of Bp26 a: Reactivity of anti Bp26 mouse serum to Bp26 synthetic biotinylated peptides: Reactivity of anti Bp26 mouse serum with synthetic biotinylated BP26-specific peptides was determined using the ELISA assay as detailed in the Materials & Methods. The absorbance readings are averages from four experiments after subtracting the mean control values (~ 0.109). b & c: Identification of collagen and vitronectin binding sites on Bp 26. Immobilized biotinylated peptides were used for identifying binding sites of collagen and vitronectin on Bp26 after incubation with collagen type I, 100 μg/ ml (b) and vitronectin, 1 μg/ ml (c). Binding was detected with peroxidase reaction using anti-collagen or anti-vitronectin Mabs (1:1000 dilution in PBS-T) followed by incubation with HRP goat anti-mouse IgG conjugate and peroxidase substrate. The reaction at A₄₅₀ was recorded with a multi-scan spectrophotometer. Control values were subtracted from the binding values