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Differences in the conditions used for the conventional and improved LuSIAs with CC81-GREMG cells
| Parameter | Conventional | Improvement |
|---|---|---|
| The number of CC81-GREMG cells | 5 × 104 cells/well | 1 × 104 cells/well |
| The number of cells in the milk | 5 × 105, 1 × 105, and 2 × 104 cells/well | 1 × 105 cells/well |
| Days of co-culture | 96 h (4 days) | 120 h (5 days) |
| Activation of B-cells by pokeweed mitogen stimulation | None | 1 µg/mL |
