Fig. 2.

SDC-1 inhibited cell growth of human colorectal carcinoma cells. pcDNA3.1 or pc-SDC-1 was transfected into SW620 and SW480 cell lines. a Cell viability was assessed by CCK-8 assay and the absorbance was measured at 450 nm. b Flow cytometry analysis to evaluate the percentage of apoptotic cells. c-e Western blot analysis was utilized to evaluate the expression levels of SDC-1, CyclinD1 and c-Myc. f-h Western blot analysis was utilized to evaluate the expression levels of SDC-1, Bax and Cleaved-Caspase-3. β-actin was used as an internal reference for normalizing the protein expression. ***p < 0.001