Fig. 1.

a Luciferase activity in MCF7 cells co-transfected with 5x kB binding sites upstream of the luciferase gene (5x NFκB-luc) and NFkB p65 and/or SIM2s. (Diagram of promoter construct is shown above for reference.) b Luciferase activity in MCF7 control or MCF7 SIM2-shRNA cells with 5x NFκB-luc. c Luciferase activity in MCF7 cells co-transfected with 5x NFκB-luc and NFkB p65 and/or SIM2s with its repression domain deleted (SIM2sΔR). d SUM159 plpcx emp (control), SUM159 plpcx SIM2s-FLAG (overexpression), MCF7 psil SCR (control), and MCF7 psil SIM2-shRNA (knockdown) were analyzed by western blot for levels of IKKα, IKKβ, phospho-p65, p65, and beta actin as a loading control. e SUM159 plpcx emp (control), SUM159 plpcx SIM2s-FLAG (overexpression), MCF7 psil SCR (control), and MCF7 psil SIM2-shRNA (knockdown) were analyzed by western blot for levels of phospho-AKTs473, pan AKT, and GAPDH as loading control. ANOVA and Student’s t test were performed to test significance. a, b, c All significant at p < 0.05, *p < 0.05. Analysis was performed via ImageJ for comparison of protein expression