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. 2019 Nov 20;14:833–842. doi: 10.1515/med-2019-0097

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© 2019 Zhendong Xiang et al. published by De Gruyter

This work is licensed under the Creative Commons Attribution 4.0 Public License.

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Mechanism dissection of how circUCK2 can suppress PCa cell growth via sponge the miR-767-5p. (A) The expression of miRNAs related to circUCK2 was determined by RT-PCR after knocking down circUCK2 in EnzS-C4-2 cells. (B) According to the prediction of Bioinformatics tools (miRBase, circRBase, and RNA22), circUCK2 has binding sites with miR-767-5p. (C) The miR-767-5p can physically interact with circUCK2. The biotinylated oligo that is complementary to the junction region of circUCK2 is mixed with the EnzR-C4-2 cell lysate, followed by purification with streptavidin beads. miR-767-5p was quantified in the pull-down complex as well as the control complex without the biotinylated oligo. (D-E) MTT assay shows that cell proliferation (D) and invasion (E) inhibition effect by overexpressing circUCK2 can be reversed by overexpressing miR-767-5p in EnzR-C4-2 cells. The data shown represent the mean of three independent experiments. *p < 0.05 by Student’s t-test for two groups or ANOVA for more than two groups.