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. 2019 Nov 26;12:10227–10237. doi: 10.2147/OTT.S226580

Figure 2.

Figure 2

CSE exposure induces EMT in SV-HUC-1 cells. (A) Western blotting detected the protein expression of epithelial markers and mesenchymal markers. (B) The mRNA expression level of SV-HUC-1 cells following CSE treatment was measured by qRT-PCR. (C) Western blotting reveals the expression of STAT3 phosphorylation in the SV-HUC-1 cells following CSE treatment for 5 days. (D) The protein expression of cell proliferation protein was measured by Western blotting. (E) Cell cycle was detected by flow cytometry. Data are expressed as mean ± SD. The values of *P<0.05, **P<0.01 show a statistical level of significance when data were compared against the control group.