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. Author manuscript; available in PMC: 2020 Apr 28.
Published in final edited form as: Nat Neurosci. 2019 Oct 28;22(12):2029–2039. doi: 10.1038/s41593-019-0515-z

Extended Data Fig. 2. Additional experiments related to Figure 3.

Extended Data Fig. 2

RNAi knockdown of Lkr in PPL1-γ1pedc DANs (MB320C-splitGAL4) does not affect 6 h water memory performance (p>0.48, n = 7-8; one-way ANOVA with Tukey’s test). RNAi knockdown of Lkr in PPL1 (TH-GAL4 and MB060B-splitGAL4) or PAM (DDC-GAL4 and R58E02-GAL4) DANs impairs 6 h performance (p<0.02, n=7-14; one-way ANOVA with Tukey’s test). b, Knockdown of Lkr after eclosion in TH-GAL4- and DDC-GAL4-labeled DANs with tubp-GAL80ts and UAS-Lkr-RNAi impairs 6 h water memory performance in thirsty flies (TH-GAL4: p<0.032; DDC-GAL4: p<0.0001; n = 12; one-way ANOVA with Tukey’s test). c, Adult brain expression of UAS-mCD8::GFP driven by MB087C-splitGAL4 (PAM-β′2a, green) and LexAop-rCD2::RFP driven by MB247-LexA::p65 (mushroom bodies, magenta). d, Adult brain expression of UAS-mCD8::GFP driven by MB296B-splitGAL4 (PPL1-γ2α′1, green) and LexAop-rCD2::RFP driven by MB247-LexA::p65 (magenta). Both brains counterstained with anti-Brp antibody (gray). Four brains were examined for each splitGAL4 and their expression patterns are consistent. Scale bars 50 μm. e, Knockdown of Lkr in PAM-β′2a (MB087C-splitGAL4) and PPL1-γ2α′1 (MB296B-splitGAL4) DANs with a second RNAi line impairs 6 h water memory performance (p<0.03, n = 12; Kruskal-Wallis test with Dunn’s multiple comparisons test). Temperature regimens shown above b and e. Box-plots: center line indicates median; box limits, upper and lower quartiles; whiskers, max to min range; dots, individual data points. See Supplementary Table 3 for statistics details.