Fig. 5.

Tam has minimal effect on the expression of selected autosomal transcripts across cortex and retina tissues. To confirm RNA-Seq findings qPCR was performed for selected gene targets. In the cortex, significant changes in Egr2, Arc, and Fos expression were evident (a). Only minimal interactions of sex with TAM treatment were evident, less than 25% differences. In the retina, very small differences with Tam treatment were observed for Banp and Map3k13. To further examine Tam effects, where specific antibodies were available, Fos and Banp protein expression in cortex (c) and retina (d) respectively were examined. No significant differences with Tam were observed. Box plots express average normalized RQ for each group (n = 3–4) ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, comparing male and female groups under the same treatment by two-way ANOVA followed by all pairwise multiple comparison procedures (Holm-Sidak method). e–f The ratio of signal intensity of Fos and Banp bands relative to β-actin bands in cortex (e) and retina (f) by WB densitometry analysis is expressed as average normalized sum intensity for each group (n = 3) ± SEM. Statistical analysis was performed by two-way ANOVA followed by all pairwise multiple comparison procedures (Holm-Sidak method)