Fig. 4.

NMN treatment significantly attenuates oxidative stress in aged CMVECs. Cellular peroxide production is significantly increased in cultured primary CMVECs derived from aged F344xBN rats as compared with cells isolated from young F344xBN rats, and it is significantly attenuated by treatment with NMN. Cellular peroxide production was assessed by measuring CM-H₂DCFDA fluorescence using a flow cytometry–based approach. Data are plotted as means ± S.E.M. (n = 6 in each group); *p < 0.05 vs. control, ^#p < 0.05 vs. aged