A. Y-Maze behavior: Rats were treated with various DHA preparations by gavage for 30 days as described in section 2.2. Y-maze behavior was determined as described in 2.3. Spontaneous alternation was calculated as the number of alternations (consecutive entries into three different arms) divided by the total possible alternations (number of arms entered minus 2) and multiplied by 100. No significant differences were found between treatment groups (n=10 per group).
B. Morris water maze: The test was carried out as described in section 2.4. Rats were trained for 4 consecutive days to locate the hidden platform. On the 5th day, the platform was removed, and the latency to reach the target area, and the time spent in the target quadrant were measured. The data were analyzed by Any-Maze software. The only significant difference observed was between the rats treated with 10 mg LPC-DHA and all the other groups during the acquisition phase (* p<0.05). There were no significant differences between groups in the probe trial. (n=10 per group).