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(a) qRT-PCR showed that, with prolonged passaging, the mRNA expression level of Col1a1 was increased, while the expression level of Col2a1 was decreased during chondrocyte monolayer culture. (b) Effects of direct coculture of hUC-MSCs with hACs (50 : 50) on gene expression of Col1a1 after 14 days, 21 days, and 28 days.
