Skip to main content
. 2019 Nov 14;2019:8169125. doi: 10.1155/2019/8169125

Figure 4.

Figure 4

Effects of T-006 on the PKA/CREB/NRF1 signaling pathway and mitochondrial biogenesis in PC12 cells. PC12 cells were treated with T-006 (30 μM) at the indicated time points, and the expression of nuclear PGC-1α and NRF1 (a) and cytoplasmic TFAM (b) were detected by Western blot. (c) Cells were incubated with T-006 (30 μM) as indicated, and the mtDNA content was quantified by quantitative real-time PCR. (d) The cells were treated with T-006 and harvested at the indicated times; the expression of phosphorylated CREB/total CREB was detected by Western blot analysis. (e) Cells were pretreated with or without PKA inhibitor H-89 (10 μM) for 1 h and then treated with T-006 (30 μM) for 30 min. The expression ratios of phosphorylated CREB/total CREB were detected by Western blot. Representative Western blot and data analysis of three independent experiments are shown. Data are represented as the means ± SD. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001, as compared to control. PC12 cells were pretreated with T-006 (30 μM) for 12 h before treatment with H-89 (10 μM) for 1 h and then challenged with 150 μM 6-OHDA for an additional 24 h; mtDNA content was quantified by quantitative real-time PCR (f). Cell viability was measured by MTT assay (g). Data from three independent experiments are represented as the mean ± SD. ###P < 0.001 compared to the control group; ∗∗P < 0.01 compared to the 6-OHDA-treated group.