Figure 4.

Behavioral assays using the Light-Dark test to measure nonmotor anxiety-like behaviors in control and permethrin (25 and 50 μg/L) exposed zebrafish larvae. (a) Time spent in the lit area traveled (average for the control group = 270 s), (b) latency to enter in the dark area (average for the control group = 67 s), (c) entries to the dark area (average for the control group = 1 entry), and (d) the number of risk assessment episodes (average for the control group = 2 episodes). The dates expressed as median ± interquartile range, analyzed by Kruskal-Wallis followed by Dunn's multiple comparison test. Groups not sharing letters are significantly different (p < 0.05).