Figure 4.

Cell size and marker expression. (a) Representative image comparing C2C12 cells shortly after dissociation of 25-day myospheres and detachment from the monolayer. The graph on the side shows the range of cell diameters. ^∗∗∗∗p < 0.0001. (b) Flow cytometric profile of Sca1 expression in cells from myospheres (25 days) and from a monolayer. (c) Semiquantitative RT-PCR profile of common quiescent satellite cell markers in differentiating and mature myospheres. Myogenin and myosin indicate the presence of differentiating cells. P: proliferating cells in monolayer. (d) Time course of CD34 expression in myospheres by western blot. (e) Caveolin-1 expression in proliferating cells and in differentiating and mature myospheres by western blot. (f) Western blot comparing hif-1α expression in proliferating cells and myospheres, with the expression of myogenic markers.