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. 2019 Nov 15;2019:7419249. doi: 10.1155/2019/7419249

Figure 7.

Figure 7

OCA pretreatment upregulated placental Nrf2 signaling during E2-induced cholestasis. All pregnant mice except controls were s.c. injected with E2 (0.625 mg/kg) once daily from GD13 to GD17. In the OCA+E2 groups, pregnant mice were administered with OCA (5 mg/kg) by gavage once daily from GD12 to GD17. All dams were sacrificed on GD18. (a, b) Placental Nrf2 was measured using immunoblot. (a) Representative gels were shown. (b) Nrf2/Lamin A/C. (c, d) Placental Nrf2 was analyzed using IHC. (c) Representative photomicrographs were shown. Original magnification: ×400. Placental mononuclear sinusoidal TGCs were stained strong positive (arrows). (d) The percentages of Nrf2-positive nuclei were evaluated among different groups. (e–g) Placental Keap1 and HO-1 were measured using immunoblot. (e) Representative gels were shown. (f) Keap1/β-actin; (g) HO-1/β-actin. Quantified data were expressed as means ± S.E.M. of six samples from six different pregnant mice. ∗∗P < 0.01.