Figure 3.
Comparison of a pulse treatment of bolus dose (bolus-pulse) vs. an extended treatment of low dose (low-extended). Bolus-pulse: Cells were incubated with unformulated CFZ at different CFZ concentrations for 2.4 h then washed and incubated in drug-free medium for additional 21.6 h making the total incubation time of 24 h. Low-extended: Cells were incubated with at 1/10th CFZ concentrations for 24 h. Cell viability was measured by the MTT assay at the end of 24 h. % cell viability was calculated by normalizing to the viability of control cells treated with the equivalent amount of vehicle for the same period of time. X-axis = CFZ concentration × exposure time (e.g., 2.4 = 1 nM for 2.4 h followed by incubation in drug-free medium for 21.6 h or 0.1 nM for 24 h). (a) Schematic description of two dosing regimens. Viability of (b) splenocytes from C57BL/6 mice, (c) dendritic cells derived from C57BL/6 mouse bone marrow and (d) B16F10 cells. *: p < 0.05, **: p < 0.01, ***: p < 0.001 and ****: p < 0.0001, by two-way ANOVA with Sidak’s multiple comparisons test.