Expression of E1A in Huh7 and HL7702 cells detected by western blot at the protein level
(a) Protein bands. (b) Relative expression levels. GP73-SphK1sR-Ad5, cells transfected with GP73-SphK1sR-Ad5 at a multiplicity of infection (MOI) of 10 for 96 h; Ad5GFP, cells transfected with Ad5GFP at an MOI of 10 for 96 h; Blank, cells without transfection. Data are expressed as mean±standard deviation (SD), n=5. Comparisons were made by one-way analysis of variance (ANOVA). *
P<0.05, vs. Ad5GFP group. N/A represents no protein expression detected