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. 2019 Oct 17;10(12):2225–2235. doi: 10.1111/1759-7714.13207

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© 2019 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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CD137L expression levels were detected by RT‐PCR and flow cytometry with staining directly or indirectly compared to isotype. (a) RT‐PCR analysis of lung cancer cell lines CD137L mRNA expression. (b) Lung cancer cell lines were each stained with mouse antihuman CD137L mAb (open histograms) or mouse IgG1, κ isotype control (shaded histograms). (c) The cells were stained with human CD137 protein (with the Fc region of human IgG) (open histograms) or human B7‐2Ig (shaded histograms), followed by staining with a secondary rabbit antihuman IgG polyclonal Ab. (d) The cells were treated with IFN‐γ (dotted line) and control (open histograms) to analyze the CD137L expression by flow cytometry. CD137L, Isotype, Control and IFN‐γ.

Inline graphic — CD137L expression levels were detected by RT‐PCR and flow cytometry with staining directly or indirectly compared to isotype. (a) RT‐PCR analysis of lung cancer cell lines CD137L mRNA expression. (b) Lung cancer cell lines were each stained with mouse antihuman CD137L mAb (open histograms) or mouse IgG1, κ isotype control (shaded histograms). (c) The cells were stained with human CD137 protein (with the Fc region of human IgG) (open histograms) or human B7‐2Ig (shaded histograms), followed by staining with a secondary rabbit antihuman IgG polyclonal Ab. (d) The cells were treated with IFN‐γ (dotted line) and control (open histograms) to analyze the CD137L expression by flow cytometry. CD137L, Isotype, Control and IFN‐γ.