Table 1.

Primers and restriction enzymes used to detect miR-146a C > G, miR-149 T > C, miR-196a2 T > C, and miR-499 A > G polymorphisms

Polymorphism	Sequence 5′ to 3′	Annealing temperature (°C)	PCR product (bp)	Restriction enzyme	Wild type	Heterozygote	Mutant
miR-146a C > G	F 5′-CATGGGTTGTGTCAGTGTCAGAGC-3′ R 5′-TGCCTTCTGTCTCCAGTCTTCCA-3′	62	147	SacI	147	147/122/25	122/25
miR-149 T > C	F 5′-TGTCTTCACTCCCGTGCTTGTCC-3′ R 5′-TGAGGCCCGAAACACCCGTA-3′	65	254	PvuII	254	254/194/60	194/60
miR-196a2 T > C	F 5′-CCCCTTCCCTTCTCCTCCAGATA-3′ R 5′-CGAAAACCGACTGATGTAACTCCG-3′	63	149	MspI	149	149/125/24	125/24
miR-499 A > G	F 5′-CAAAGTCTTCACTTCCCTGCC-3′ R 5′-ATGTTTAACTCCTCTCCACGTGATC-3′	65	145	BclI	145	145/120/25	120/25