Table 2.
Statistic comparison of apoptotic sperm markers between control group and teratozoospermic group
| Control group (n=30) Mean ± SD |
Teratozoospermic group (n=60) Mean ± SD |
p value | |
|---|---|---|---|
| AO/EB staining assay | |||
| Normal spz (%) | 70.08±21.8 | 21.92±15.76 | 0.001b |
| Early apoptotic spz (%) | 25.11±17.25 | 29.98±12.16 | 0.311 |
| Late apoptotic spz (%) | 3.75±6.98 | 30.47.±16.21 | 0.001b |
| Necrotic spz (%) | 1±3.16 | 16.53±15.15 | 0.003b |
| Caspase assay | |||
| Casp+/PI− (%) | 54.85±12.36 | 28.09±10.62 | 0.001b |
| Casp+/PI+ (%) | 43.79±11.95 | 69.34±10.28 | 0.001b |
| Casp−/PI+ (%) | 1.42±0.98 | 2.3±1.08 | 0.011a |
All values are expressed as mean ± standard deviation and analyzed using the paired t test
spz spermatozoa
aSignificant difference with control group (p<0.05)
bHighly significant difference with control group (p<0.01)