Fig. 6. KLF5 silencing inhibits cardiomyocyte apoptosis.

a Restoration of reduced cell viability by silencing KLF5 in NRVMs treated with 200 μM H₂O₂ for 4 h (n = 6). *P < 0.05, **P < 0.01 by one-way ANOVA analysis with Tukey’s multiple comparison test. Data are presented as mean ± SEM. b Statistical results of TUNEL-positive cells per field (n = 5). **P < 0.01 by one-way ANOVA analysis with Tukey’s multiple comparison test. Data are presented as mean ± SEM. c Representative images of TUNEL staining of NRVMs for DNA defragmentation showing the apoptotic cells (scale bar: 60 μm). Data are presented as mean ± SEM. d KLF5 silencing suppressed Bax elevation in NRVMs treated with H₂O₂ (n = 5). *P < 0.05 by one-way ANOVA analysis with Tukey’s multiple comparison test. Data are presented as mean ± SEM. e Downregulation of KLF5 protein level by LncR-TUG1 knockdown in NRVMs (n = 5). Note that the effect of LncR-TUG1 knockdown was prevented by AMO-9. *P < 0.05 by one-way ANOVA analysis with Tukey’s multiple comparison test. Data are presented as mean ± SEM. f Decrease of H₂O₂-induced upregulation of KLF5 protein level by LncR-TUG1 knockdown in NRVMs (n = 4). **P < 0.01 by one-way ANOVA analysis with Tukey’s multiple comparison test. Data are presented as mean ± SEM.