Fig. 2. BAG5 knockdown enhances mitophagy.
a Representative flow cytometry data of U2OS mito-mKeima cell lines also expressing GFP-Parkin or GFP-ParkinC431S that were untreated or treated with 20 μm CCCP for 4 h after transfection with non-targeting, BAG5, or PINK1 siRNA. Mitophagy was assessed as the percentage of cells displaying an elevated 561/405 nm ratio as captured in the upper gate as we have previously described22,23. b Quantification of average percentage of mitophagy from three independent experiments. Statistical significance was determined by a one-way ANOVA with Tukey’s post hoc test (**p < 0.005, ****p < 0.0001).