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. 2019 Dec 2;10(12):905. doi: 10.1038/s41419-019-2140-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a HEK293T cell lysates co-transfected of NR2B (or NR2B-S2A, NR2B-S2E), PKCα, PICK1, and GFP plasmids were immunoblotted. The expression of PKCα decreased significantly when co-transfected with PICK1 and NR2B-S2E (**). The expression intensity of NR2B compared to GFP was not significantly different among three groups. The expression intensity of PKCα compared to GFP decreased significantly in NR2B-S2E group. Average intensity of PKCα/GFP: NR2B, 0.08 ± 0.01; NR2B-S2A, 0.09 ± 0.02, P = 0.1046, NR2B-S2E, 0.008 ± 0.0003, P = 0.0007. NR2B-S2A: The non-phosphorylated form of NR2B; NR2B-S2E: phosphorylated-mimicking form of NR2B. b HEK293T cell lysates co-transfected of NR2B (or NR2B-S2A, NR2B-S2E), PKCα and PICK1 plasmids were immunoprecipitated (IP) and immunoblotted (IB). The control was incubated with IgG antibody. NR2B, PKCα and PICK1 combined together as a triad complex. Average intensity of PKCα/Input of WT, S2A, and S2E group, n = 3: NR2B: 0.90 ± 0.16; 1.03 ± 0.20, P = 0.5958; 0.5958pnt, P = 0.2100; S2A: 0.77 ± 0.15, P = 0.1606; 0.9106776, P = 0.1606, P = 0.4933; 0.55 ± 0.15, P = 0.4933; S2E: 0.86:4933; 0.87 ± 0.13, P = 0.5732; 0.62 ± 0.06, P = 0.09.