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. 2019 Oct 16;294(48):18057–18068. doi: 10.1074/jbc.RA119.008925

Figure 2.

Figure 2.

Overexpression of TSG101 improves animal survival and cardiac function and reduces inflammation upon endotoxin challenge. A, experimental procedure and biochemical assays for LPS (10 μg/g) treatment in WT and TG mice. wk, week. B, Western blots and quantification analysis showing the expression of TSG101 in hearts of WT and TG mice. GAPDH was used as a loading control for total protein. n = 6 for all groups. *, p < 0.05 versus WT. C, survival curve of WT and TG mice when challenged with LPS (10 μg/g) and monitored over 5 days. n = 8 for WT, n = 7 for TG. *, p < 0.05 versus WT. D–F, cardiac function in WT and TG mice subjected to PBS or LPS treatment was determined by echocardiography. n = 4 for PBS WT, n = 5 for PBS TG, n = 5 for LPS WT, n = 7 for LPS TG. * and #, p < 0.05 versus PBS WT; &, p < 0.05 versus LPS WT. G and H, levels of the pro-inflammatory cytokines TNFα (G) and IL-6 (H) in WT and TG mice subjected to PBS or LPS treatment were determined by qRT-PCT. n = 4 for all groups. *, p < 0.05 versus PBS WT; #, p < 0.05 versus PBS TG; &, p < 0.05 versus LPS WT. I and J, levels of the pro-inflammatory cytokines TNFα (I) and IL-6 (J) in WT and TG mice subjected to PBS or LPS treatment were measured by ELISA. n = 6 for all groups. *, p < 0.05 versus PBS WT; #, p < 0.05 versus PBS TG; &, p < 0.05 versus LPS WT.