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. 2019 Oct 25;294(48):18306–18323. doi: 10.1074/jbc.RA119.010348

Figure 6.

Figure 6.

ARID3C was identified as one of the major β-catenin downstream targets in controlling ovarian cancer cell proliferation. A, comparison of steady-state RNA levels are shown as reads per million (rpm), from pre-injected OVCAR5 cells, PTPRR-WT expressed OVCAR5 cells, and PTPRR-DA expressed OVCAR5 cells. Dashed lines, 2⋀0.5-fold changes. All genes above this threshold are represented as red dots. The average of two replicates is shown. B, KEGG pathways commonly containing significantly changed DEGs. C, comparison of steady-state RNA levels are shown as reads per million, from tumor cells derived from OVCAR5 cell, PTPRR-WT–expressed OVCAR5 cell, and PTPRR-DA–expressed OVCAR5 cell injection. Dashed lines, 2⋀0.5-fold changes. All genes above this threshold are represented as red dots. The average of three replicates is shown. D, heat map of significantly changed genes in both cell line RNA-Seq and tumor tissue RNA-Seq. E, overlap of significantly changed genes from ovarian cancer cell line RNA-Seq and tumor tissue RNA-Seq with targeted genes from the CTNNB1-CHIP promoter array (GSE21151). F, representative qRT-PCR analysis detecting ARID3C expression level in cells and tumors from OVCAR5 cell, PTPRR-WT–expressed OVCAR5 cell, and PTPRR-DA–expressed OVCAR5. The experiment was done in triplicate. Data are shown as means ± S.E. (error bars). G, representative qRT-PCR analysis to detect the PBXIP1 expression level in cells and tumors from OVCAR5 cells, PTPRR-WT–expressed OVCAR5 cell, and PTPRR-DA–expressed OVCAR5. The experiment was done in triplicate. Data are shown as means ± S.E. H, representative qRT-PCR analysis detecting DVL3 expression level in cells and tumors from OVCAR5 cells, PTPRR-WT–expressed OVCAR5 cells, and PTPRR-DA–expressed OVCAR5. The experiment was done in triplicate. Data are shown as means ± S.E. I, ChIP-PCR assay confirming the binding of CTNNB1 to the promoter region of ARID3C in OVCAR5 and PTPRR-DA–expressed OVCAR5 cells but not PTPRR-WT–expressed OVCAR5 cells. J, representative qRT-PCR analysis detecting ARID3C expression level in OVCAR5 cells and PTPRR-WT–expressed OVCAR5 cells with or without ARID3C shRNA knockdown. The experiment was done in triplicate. Data are shown as means ± S.E. K, cell proliferation assay on OVCAR5 and PTPRR-WT–expressed OVCAR5 cells with and without ARID3C knockdown. The experiment was done in hexatriplicate. Data are shown as means ± S.E. n.s., not significant.