Figure 3.

Time-dependent formation of long-lived Chd1–nucleosome complexes. For these experiments, 40 or 320 nm Chd1 was incubated with 10 nm FAM-labeled 40N40 nucleosomes and challenged with 1 μm unlabeled 26N33 nucleosomes. The apparent on-rates, fit with single exponential equations (dotted traces), were 1.0 ± 0.3 min⁻¹ (320 nm Chd1, 1 min), 0.27 ± 0.08 min⁻¹ (40 nm Chd1, 1 min), and 0.18 ± 0.07 min⁻¹ (40 nm Chd1, 2 h). For the 1-min incubations with competitor, the values are averages of two separate experiments, with error bars indicating the ranges. For the 2-h incubation with competitor, the data are the mean values ± S.D. from four experiments.