Figure 4.

Binding capacities of the RGD-integrin family to the WT, disease causing (WC), and RGE knock-out fragments. For all panels, x axis denotes concentration of purified fibrillin-1 fragment. A–E, cell adhesion of endogenous and transfected integrins in SW480 cells. All conditions were completed in the presence of blocking antibodies against all integrins but one of interest. F, cell adhesion of integrin αvβ1 in HDF, completed in the presence of P1D6, ALULA, and AXUM2. H, cell adhesion of integrin αIIbβ3 in human platelets isolated from whole blood, completed in the presence of L230 and P5D2. Data represents mean of 2 biological replicates ± S.E.